Last data update: May 13, 2024. (Total: 46773 publications since 2009)
Records 1-2 (of 2 Records) |
Query Trace: Chafin DL[original query] |
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Stabilities of intact hemoglobin molecules and hemoglobin peptides in dried blood samples
Adam BW , Haynes CA , Chafin DL , De Jesus VR . Clin Chim Acta 2013 429 59-60 Sickle cell diseases are inborn blood disorders caused by the presence of an abnormal form of hemoglobin, hemoglobin S (HbS). Mortality from sickle cell disease during the first 3 to 4 years of life can be virtually eliminated by newborn screening and appropriate follow-up and treatment [1]. Three sickle cell diseases (sickle cell anemia, sickle hemoglobin C disease and sickle β thalassemia) are included in the United States' recommended uniform newborn screening panel [2]. HbS is the newborn screening marker for all three disorders. | The Newborn Screening Quality Assurance Program of the Centers for Disease Control and Prevention (CDC) prepares and validates dried-blood spot (DBS) proficiency testing materials to assist laboratories with monitoring the performance of their newborn screening tests [3]. As part of routine evaluations of marker stabilities in DBS samples, we used measurements by high performance liquid chromatography (HPLC) and liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) methods to compare the stabilities of HbA and HbS in DBSs stored for predetermined intervals at elevated temperature (37°C) and low (<30%) or high (>50%) humidity. The objectives of these studies were to measure separately the contributions of heat and humidity to the degradation of HbS and HbA in DBS samples and to evaluate the level of concordance between results from the two analysis methods used. |
Stabilities of hemoglobins A and S in dried blood spots stored under controlled conditions
Adam BW , Chafin DL , De Jesus VR . Clin Biochem 2013 46 (12) 1089-1092 OBJECTIVE: We aimed to measure separately the contributions of heat and humidity to changes in levels of hemoglobins A and S in dried-blood-spot (DBS) samples. DESIGN AND METHODS: We stored paired sets of DBSs at 37 degrees C for predetermined intervals in low-humidity and high-humidity environments. Hemoglobin A and S levels of all samples in each complete set were measured in a single high performance liquid chromatography run. RESULTS: During the one-month storage intervals, both hemoglobin species lost about 35% of initial levels in low-humidity storage and almost all of initial levels in high-humidity storage. CONCLUSIONS: Minimizing both humidity and temperature in the transportation and storage environments of DBS samples is essential to maintaining the integrity of the hemoglobin tetramer molecules. |
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